Dr. Rathnagiri Polavarapu

President & CEO Genomix Biotech Inc & Genomix Molecular Diagnostics Pvt. ltd

Address:

5-36/207, Prasathi Nagar, kukatpally
Hyderabad, Andhra Pradesh- 500072, INDIA.
Ph: 9849114520, E-Mail: giri@genomixbiotech.com

Education: Ph.D

Professional experience:

Molecular biology, Cell biology, Pathology, Oncology, Signal transduction pathways, functional Genomics, Proteomics, Gene expression and regulation research, Therapeutic intervention of Disease processes, Micro-arrays, Developing rapid detection Diagnostic kits and customer care

Position Held
Duration
President & CEO Genomix Biotech Inc, USA and  Genomix Molecular Diagnostics Pvt. Ltd, India.
2007-Till date

Director of Operations & Acting Managing Director
MWG Biotech Inc. High Point, NC, USA

6/2005- 8/2006

Research Biologist Lead GS-14
Division of Bioterrorism; NCID; CDC/Homeland Security; USA

2/2003 to 6/2005
  • Effectively managed, Directed the Oligonucleotide (5,000 oligos per a day), Si RNA, Duel probe Production and High throughput Sequencing units (2,000 reads per a day).
  • Played key role to receive “Certificate of Appreciation” from Secretary of Homeland Security” for successfully deploying rapid detection molecular methodologies and for bench marking rapid detection of several crucial agents of bioterrorism like Bacillus anthracis, Brucella species, Yersinia pestis, Francisella tularensis, Clostridium Botulinum, variola virus etc at City of Huston jurisdiction.
  • Trained several associates regarding protocols for rapid detection of environmental samples, and clinical samples using serological, biochemical and conventional methodologies like ELISA and other assays including West Nile virus etc. Collaborated with National labs and CDC in genotyping select agents
Position Held Duration

Director; Emory  DNA Core Facility & Professor of Medicine and Biochemistry; Emory University, Atlanta, GA, USA

7/1997-10/2002
  • Initiated and Established a molecular Core facility that served for more than 400 principal investigators regarding High throughput automated DNA sequencing, Microarray technology and RT-PCR etc.
  • Involved in designing and execution of projects and collaborated with several investigators regarding their functional genomics studies and helped in grant writing.
Position Held Duration

Assistant Professor
Department of Pharmacology and Molecular Core Lab Manager. PENN State University; Hershey, PA, USA

1993- 1997
  • Initiated own research in biomedical fields of disease processes and obtained research grants and published in peer-reviewed journals. Trained several students for Ph.D. thesis and mentored towards their graduation.
Position Held Duration

Visiting Fellow & Visiting Scientist
Section on Immunology; NIAAA, NIH, USA

1984-1993
  • Published several papers (a total of 40) in collaboration with other investigators in wide range of biomedical research fields including cancer, immunology and toxicology.
  • Cloned and studied expression and regulation of several gene families including ADH, Calcineurin, Stannin etc.

Projects handled:

  • Cloning and expression of human Alcohol dehydrogenases: NIAAA, NIH, USA (1984-1987)
  • Role of different Isoforms of Calcineurin and their expression and  tissue specific regulation NIAAA, NIH, USA (1987-1993)
  • Multidrug resistance in child hood cancers and role of different pathways in therapeutic intervention of disease processes. Four Diamonds’ grant, PSU, Hershey, PA USA (1993-1997)
  • MDR phenotypes and developing low density Gene chip microarrays. VA Hospital, Atlanta, GA (1997-2000)
  • Instrumentation grant for High through put sequencing, Microarrays and Laser captured Microscopy. VA Hospital/ Emory University, Atlanta, GA USA (1999-2002)
  • Developing rapid inexpensive instrumentation for the detection of Malaria and other diseases., SBIRI, DBT, India (2009-2012)

Salient achievements:

During the past 30 years held several positions at NIH, and faculty at different universities including Penn State, Emory and CDC.  Presently holding Professorship at NTR Helath Sciences and Nagarjuna Universities in the dept of Biochemistry and Biotechnology.

  • Managerial Skills: Managed three shifts of a total 47 employees directly.  Strong Customer Relationship with positive interaction, trouble shooting and provides extensive technical advice.
  • Cell Biology: Cell and tissue culture, monoclonal and polyclonal production, microbiology & immunology techniques
  • Proteomics: Protein purification and characterization, enzymology, 2D-PAGE, Phosphor-protein analyses, cell culture
  • Functional Genomics: Molecular cloning, gene mapping, cDNA and Genomic DNA library construction, ESTs, High throughput mega sequencing projectsHigh-density gene chip techniques.
  • Biopharmaceuticals & Molecular diagnostics: Design & construction of therapeutically significant genes in to vectors and express in different systems using large biofermentors. Preparation of pathogen specific immuno/molecular rapid detection kits.

Top five research papers:

  1. Muramatsu, T., R. Polavarapu, S. Higuchi, and R. L. Kincaid. (1992). Molecular cloning of a calmodulin-dependent phosphatase from murine testis. Identification of developmentally expressed non-neural isozyme. Procedings of National Academy of Sciences. USA.  89, 529-533.
  2. Polavarapu, R., C. A. Marietta, S. Higuchi, and R. L. Kincaid. (1992). Molecular and phylogenetic analysis of calmodulin-dependent phosphatase (calcineurin) catalytic subunit genes. DNA and Cell Biology  11, 415-424.
  3. R. Surabattula, M. Nagasundram, R. Polavarapu and K.R.S. Sambasivarao (2010). Improvement of Recombinant CHO cell growth, comparison of Growth characteristics and erythropoietin production, International Journal of Biotechnology and Biochemistry, vol. 6 (7),  1003-1022
  4. Rambabu Surabattula,  K.R.S. Sambasivarao and Ratnagiri Polavarapu (2011). An optimized process for expression, scale-up and purification of recombinant erythropoietin produced in CHO cell cultures, Research in biotechnology, vol.2, no.3, pp.58-745.
  5. Rambabu  Surabattula,  Manjupradeep  Vejandla,  Konrad Faulstich and Ratnagiri Polavarapu (2012). Simple, rapid, inexpensive platform for the diagnosis of Malaria by loop mediated isothermal amplification (submitted)

Awards and recognitions:


NIH Research Fellowship, Visiting Scientist,
Recognition from Dept of  Homeland security for successfully launching Bioterrorism program especially Brucella and Francesella at CDC, USA
Technologies developed: (Like diagnostic kits/vaccines/patents etc list only)
LAMP instrumentation for the detection of Malaria and other pathogens (Patent submitted)
Nanoparticle based pneumonia vaccine (Patent pending)

Professional affiliations:


President & CEO; Genomix Biotech USA and Genomix Molecular Diagnostics Pvt Ltd
Professor & Associate Dean for Research, Katuri Medical College; NTR Health Science University, AP.