Dr. Puran Chand



Brucellosis Laboratory
College Central Laboratory
College of Veterinary Sciences
Lala Lajpat Rai University of Veterinary and Animal Sciences
Hisar-125 004
Phone (O) :    +911662289320       Fax No.: +911662284312
Mobile :           09255120256         E-mail: cpuran@gmail.com


(1) B.V.Sc & A.H. and M.V.Sc. from G.B. Pant University, Uttar Pradesh
(2) Ph.D. from University of Surrey, England, U. K.

Professional experience:

Owing to immense public health concerns and economic impact of brucellosis to ruminant livestock, the work was initiated in my laboratory way back in late eighties. Initially when I started working on this nationally important problem the information was scanty in India. I had several successful isolations of Brucella abortus organisms in my lab and established internationally recognized laboratory protocols for isolation and characterization of this very important zoonotic pathogen. Though quite a few conventional antigen-antibody based assays were available, a need was felt to have more sensitive, specific, rapid and reliable diagnostic assay which could be used for detection of Brucella in a variety of clinical specimens. In an effort to develop on spot assay, a collaborative research was established with National Institute of Immunology under the guidance of Professor G.P. Talwar. This collaboration resulted into development of a very useful spot test. Based on my substantive contribution from India as evidenced by quality international publications I was invited as an Indian expert in the meeting jointly organized by FAO/WHO in China in 1992. My laboratory continued to expand work on different dimensions of brucellosis under which a study was conducted to assess the impact of brucellosis in breeding rams. For the first time we could isolate Brucella melitensis from clinical cases of breeding rams. Subsequently an excellent diagnostic assay for detecting Brucella antibodies in sheep milk by ELISA was developed. Thus having gained considerable experience and insight into Brucella disease complexity, we were in a position to develop a model for control of brucellosis at farm level. The idea of this control model was proposed to an organized government farm which gained acceptance at the highest level of National policy making body. Based on laboratory’s scientific advice, the said farm adopted the policy of vaccination in sheep against this dreaded disease. Due to success obtained in this organized farm having around 7000 animals, I have been an experienced expert in several policy making bodies of the State and Central Governments. Considering long experience in the field I was invited to develop a national training for Continuing Veterinary Education Program of Government of India under aegis of Veterinary Council of India. More recently, on the advice and strong recommendations from my laboratory the Animal Husbandry Commissioner, Govt. of India convened an Experts Consultation Meeting in which the existing brucellosis control program for bovines was revised and it was resolved and decided to include adult animal vaccination, besides calf-hood vaccination. On my recommendations, reduced dose of S19 vaccine by conjunctival route of inoculation was accepted to vaccinate adult animals.

Projects handled:

  1. Etiology, diagnosis and control of abortions in domestic animals funded by Govt. of Haryana
  2. Testing of sheep flock for brucellosis funded by Ministry of Agriculture, Govt. of India
  3. Diagnostic services related to abortions in domestic animals - Self Financed Scheme as approved by the University and Govt. of Haryana
  4. Continuing Veterinary Education Program on Training of Diagnosis of Brucellosis- funded by Govt. of India through Veterinary Council of India 

Salient achievements:

  1. Developed Dot-ELISA to detect Brucella antibody in blood of cattle, buffaloes, sheep and goats using anti-species tracer methodology or Protein-A system
  2. Developed Co-agglutination and counter immuno-electrophoresis methods to detect Brucella antigen in stomach contents of aborted fetus.
  3. Developed ELISA for detection of Brucella antibody in milk of lactating ewes.
  4. Several isolations of Brucella abortus from buffaloes and cows and Brucella melitensis from sheep and goats.
  5. Isolation of Brucella abortus from humans.
  6. Published more than 60 papers in national/international peer reviewed Journals.
  7. Addressed many plate farms of Central and State Governments in respect of control of brucellosis in animals

Top five research papers:

  1. Chand P. and Chabbra R. (2013). Herd and individual animal prevalence of bovine brucellosis with associated risk factors on dairy farms in Haryana and Punjab in India. Tropical Animal Health Production 45: (http://dx.doi.org/10.1007/s11250-013-0362-y).
  2. Chand, P., Chhabra, R., Singh, I., Banger, R. and Jangra, S. (2013). Control of brucellosis on an infected Murrah buffalo farm with reduced dose of Brucella abortus S19 vaccine administered by conjunctival route in adult animals. Indian Journal of Animal Sciences 83: 351-356.
  3. Chand, P., Rajpuruhit, B.S. and Batobyal, A. (2012). Control of brucellosis at an endemically infected sheep breeding farm in India.  Indian Journal of Animal Sciences 82: 343–347.
  4. Kumar, M. and Chand, P. (2011). Improvement in the diagnosis of Brucella abortus infections in naturally infected water buffaloes (Bubalus bubalis) using an ELISA with a protein-G-based indicator system. Tropical Animal Health Production, 43:1493–1499.
  5. Chand, P., Rajpurohit, B.S., Malhotra, A. K. and Poonia, J.S. (2005). Comparison of milk-ELISA and serum-ELISA for the diagnosis of Brucella melitensis infection in sheep. Veterinary Microbiology 108: 305-311.

Awards and recognitions:

  1. Commonwealth Scholarship Award by Commonwealth Commission, U.K.
  2. Reviewer of Tropical Animal Health Production journal.
  3. Expert from India in OIE/FAO Meeting in China for developing strategies for diagnosis and control of brucellosis in Asia 
  4. Expert on Bovine Brucellosis Control Program of Govt. of India
  5. Expert on Brucellosis Control Program of Govt. of Haryana
  6. Expert in Working Group of ICAR-NDDB-ILL for validation of Diagnostic Kits for brucellosis developed indigenously by IIL, Hyderabad and PD_ADMAS, Bangalore
  7. Expert/Advisor to IIL, Hyderabad for validating reduced dose of Brucella abortus S19 vaccine for adult vaccination
  8. Expert/Advisor to NDDB for implementing S19 vaccination for adult animals
  9. Ganti. A Sastri Award
  10. Dr. C. M. Singh Award
  11. Expert on Brucellosis on Continuing Veterinary Education Program of Indian Government
  12. Expert/Advisor to Punjab State Farmers Commission, Govt. of Punjab

Technologies developed:

  1. Protein-G based ELISA for diagnosis of brucellosis in buffaloes: Patent has been filed as under:
  2. Patent Application #1064/DEL/2010 dated 6.5.2010 on “Protein-G based Technology for the diagnosis of brucellosis in buffaloes” (File Ref. # 09036)

Professional affiliations:

  1. Life member of  Indian Association of Advancement of Veterinary Research
  2. Life member of  Indian  Veterinary Associations
  3. Life member of Indian Association of Veterinary Microbiology, Immunology and Infectious diseases
  4. Life member of Indian Association of Immunology and Immunopathology
  5. Life member of Association of Microbiologists of India